Expression of Xylella fastidiosa fimbrial and afimbrial proteins during the biofilm
نویسنده
چکیده
9 10 11 Caserta, R. ; Takita, M. A.; Targon, M. L.; Rosselli-Murai, L. K.; de Souza, A. P.; Peroni, L.; Stach-Machado, D. R.; Andrade, A.; Labate, C. A.; Kitajima, E. W.; Machado, M.A.; de Souza, A. A. 1. Centro APTA Citros Sylvio Moreira/IAC, Rodovia Anhanguera Km 158, Cordeirópolis – 7 SP, BRAZIL, Zip code: 13490-970. 8 2. Universidade Estadual de Campinas/UNICAMP, Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolução, Instituto de Biologia, PO Box 6010, Campinas – SP, BRAZIL, Zip code: 13083-970. 3. Universidade Estadual de Campinas/UNICAMP, Laboratório de Imunologia Aplicada, 12 Departamento de Microbiologia e Imunologia, Rua Monteiro Lobato s/n, Campinas – SP, 13 BRAZIL, Zip code:13083-970. 14 15 16 17 18 19 20 21 22 23 24 25 4. Escola Superior de Agricultura “Luiz de Queiroz”/USP, Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, P.O. Box 83, Piracicaba SP, BRAZIL, Zip code: 13400-970. 5. Escola Superior de Agricultura “Luiz de Queiroz”/USP, Núcleo de Apoio à Pesquisa em Microscopia Eletrônica aplicada à Pesquisa Agropecuária (NAP/MEPA), Piracicaba SP, BRAZIL, Zip code: 13418-900.
منابع مشابه
EFFECTS OF FIMBRIAL (FIMA, FIMF) AND AFIMBRIAL (XADA, HXFB) ADHESINS ON THE ADHESION OF XYLELLA FASTIDIOSA TO SURFACES Project Leader:
We investigated the role of fimbrial and afimbrial adhesions in the attachment of Xylella fastidiosa (Xf) to grape. We have individually disrupted FimA, FimF, XadA, and HxfB to assess their role in adhesion to plants and in the disease process. We performed adhesion assays using each mutant and wild-type separately as well as a combination of two mutants at one time to observe the phenotypes of...
متن کاملLocalization and characterization of Xylella fastidiosa haemagglutinin adhesins.
Xylella fastidiosa is a gram-negative, xylem-inhabiting, plant-pathogenic bacterium responsible for several important diseases including Pierce's disease (PD) of grapevines. The bacteria form biofilms in grapevine xylem that contribute to the occlusion of the xylem vessels. X. fastidiosa haemagglutinin (HA) proteins are large afimbrial adhesins that have been shown to be crucial for biofilm for...
متن کاملIn vitro Determination of Extracellular Proteins from Xylella fastidiosa
The phytopathogen Xylella fastidiosa causes economic losses in important agricultural crops. Xylem vessel occlusion caused by biofilm formation is the major mechanism underlying the pathogenicity of distinct strains of X. fastidiosa. Here, we provide a detailed in vitro characterization of the extracellular proteins of X. fastidiosa. Based on the results, we performed a comparison with a strain...
متن کاملGene expression profile of the plant pathogen Xylella fastidiosa during biofilm formation in vitro.
A biofilm is a community of microorganisms attached to a solid surface. Cells within biofilms differ from planktonic cells, showing higher resistance to biocides, detergent, antibiotic treatments and host defense responses. Even though there are a number of gene expression studies in bacterial biofilm formation, limited information is available concerning plant pathogen. It was previously demon...
متن کاملSurface Physicochemical Properties at the Micro and Nano Length Scales: Role on Bacterial Adhesion and Xylella fastidiosa Biofilm Development
The phytopathogen Xylella fastidiosa grows as a biofilm causing vascular occlusion and consequently nutrient and water stress in different plant hosts by adhesion on xylem vessel surfaces composed of cellulose, hemicellulose, pectin and proteins. Understanding the factors which influence bacterial adhesion and biofilm development is a key issue in identifying mechanisms for preventing biofilm f...
متن کامل